ABSTRACT
O objetivo deste trabalho foi determinar a atividade antimicrobiana do carvacrol e sua combinação com tiabendazol no controle de fungos patogênicos deteriorantes de frutas (Colletotrichum gloesporioides, Fusarium solani e Alternaria alternata). O carvacrol apresentou uma concentração inibitória mínima (CIM) de 282 a 563 μg mL-1 para os fungos testados. Quando avaliado em conjunto com o tiabendazol apresentou efeito aditivo contra C. gloesporioides e F. solani (FICI 0,5 e 1,0, respectivamente) e sinérgico contra a A.alternata (FICI 0,1). Houve redução da CIM do carvacrol de 50 a 88%. Este estudo mostra o potencial do uso.
Subject(s)
Alternaria/drug effects , Colletotrichum/drug effects , Fungicides, Industrial/administration & dosage , Fungicides, Industrial/analysis , Fungi/drug effects , Fusarium/drug effects , Food Microbiology , Thiabendazole/administration & dosage , Drug SynergismABSTRACT
Abstract The β-Glucans content has straight influence on the quality of malt and beer, mainly during the filtration step. Barley presenting high β-Glucan content demands longer germination time at malting. The application of commercial β-Glucanase is an alternative to accelerate the process and preserve the quality of malt. This work aimed to evaluate the effect of commercial β-Glucanase addition in malt produced within reduced germination time (64 h). Micro-malting was conducted with BRS-Caue and Elis barley cultivars at germination time 64 h and 96 h. The β-Glucanase concentration applied were 0, 25, 50 and 100 mg.kg-1. Barley, malt and wort samples were analyzed to check their physical-chemical features. Beers were produced with BRS-Caue malt and the physical-chemical and sensory attributes were analyzed. The commercial enzyme addition in BRS-Caue and Elis (64 h), at concentration 25 and 50 mg.kg-1, resulted in wort presenting proper β-Glucan content (≤ 178 mg.L-1). The beer produced with malt germinated for 64 h and added with 50 mg.kg-1 of β-glucanase was the one showing the largest number of physical-chemical and sensory parameters similar to the beer made with malt germinated for 96 h (conventional process). Commercial β-glucanase application in malt allowed accelerating the malting process without affecting the quality of the malt for beer production.
Subject(s)
Brewery , Germination/drug effects , Seedlings , beta-Glucans/administration & dosage , Identity and Quality Standard for Products and ServicesABSTRACT
Soil fungi were evaluated regarding their ability to degrade lignin-related compounds by producing the ligninolytic enzymes. Lignosulfonic and tannic acids were used as sole carbon sources during 30 days under microaerobic and very-low-oxygen conditions. The fungi produced lignin-peroxidase, manganese-peroxidase and laccase . Expressive degradations was observed by C18 reversed-phase HPLC, indicating the biodegradation potential of these fungi, showing more advantages than obligate anaerobes to decontaminate the environment when present naturally.
Fungos isolados de solo foram avaliados quanto à habilidade em degradarem compostos derivados de lignina pela produção de enzimas ligninolíticas. Os ácidos lignosulfônicos e tânico foram usados separadamente como única fonte de carobono para cultivo dos fungos em 30 dias sob condições microaeróbias. Os fungos foram capazes de crescer e usar tais compostos como fonte de carbono e mostraram produção de lignina-peroxidase, manganês-peroxidase e lacase. Degradações expressivas dos ácidos lignosulfônico e tânico foram verificadas por Cromatografia Liquida de Alta Eficiência (CLAE), indicando grande potencial de uso em processos de biorremediação de macromoléculas aromáticas similares à lignina em ambientes naturais sob condições baixas de oxigenação.